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Description
Research Area
Cardiovascular Research
Images & Validation
−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | The bioactivity of rh-aFGF protein was determined in a BALB/c 3T3 mouse fibroblasts cell proliferation assay. The ED50 of each lot is between 50-200 pg/mL. |
| Target | FGF1 |
| Tag | Tag free |
| MW | 15.8 kDa |
| Purity | >98% as determined by SDS-PAGE. |
| Protein Sequence | Phe 16 - Asp 155 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−FGF1, AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1
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The purity of rh-aFGF was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
Documents Download
Datasheet
Product Information
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
FGF1 Recombinant Protein (orb1228197)
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