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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | The bioactivity of rh-aFGF protein was determined in a BALB/c 3T3 mouse fibroblasts cell proliferation assay. The ED50 of each lot is between 50-200 pg/mL. |
| Target | FGF1 |
| Tag | Tag free |
| MW | 15.8 kDa |
| Purity | >98% as determined by SDS-PAGE. |
| Protein Sequence | Phe 16 - Asp 155 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−FGF1 (NM_000800) Human Recombinant Protein [orb3035943]
>95% as determined by SDS-PAGE and Coomassie blue staining
15.9 kDa
100 μg, 1 mgHuman FGFa (154AA) Protein [orb1471724]
Unconjugated
Greater than 95% as determined by reducing SDS-PAGE.
17.3 KDa
Mammalian
10 μg, 50 μgRecombinant Human FGFa Protein [orb3002211]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 17.3 KDa. Observed: 17 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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The purity of rh-aFGF was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
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Protocol Information
FGF1 Recombinant Protein (orb1228197)
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