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FGF acidic Recombinant Protein

SKU: orb1230621

Description

FGF acidic Recombinant Protein

Research Area

Stem Cell & Developmental Biology

Images & Validation

Key Properties

SourceE. coli
Biological ActivityAssay 1: The ED50 was determined by the dose - dependent stimulation of thymidine uptake by 3T3 cells in the presence of heparin is ≤ 0.5 ng/mL corresponding to a specific activity of ≥ 2 x 106 units/mg.Assay 2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.2 ng/mL in the presence of 10 μg/mL heparin, corresponding to a specific activity of ≥ 5 x 106 units/mg.
TargetFgf1
PurityGreater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Protein SequenceMFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

Storage & Handling

StorageThe lyophilized FGF acidic recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted FGF acidic is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing FGF acidic recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Form/AppearanceLyophilized
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Fam, Fgfa, Dffrx, Fgf-1, Fibroblast growth factor 1, Acidic fibroblast growth factor, FGF-1

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
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FGF acidic Recombinant Protein (orb1230621)

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