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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 0.5 ng/ml in the presence of 10 ug/ml heparin, corresponding to a specific activity of ≥ 2 x 106 units/mg. |
| Target | Fgf1 |
| Purity | Greater than 95% by SDS-PAGE gel and HPLC analyses. |
| Protein Sequence | MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D |
Storage & Handling
−| Storage | The lyophilized FGF-acidic recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted FGF-acidic is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing FGF-acidic recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperat.ure. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Fam, Fgfa, Dffrx, Fgf-1, Fibroblast growth factor 1, Acidic fibroblast growth factor, FGF-1
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Recombinant Human Fibroblast Growth Factor- acidic (rHuaFGF ) [orb1495079]
>95% by SDS-PAGE and HPLC analyses.
Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 140 amino acids.
Escherichia coli.
1 mg, 10 μg, 50 μg
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_034327.1 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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