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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Determined by its ability to stimulate the proliferation of murine NIH-3T3 cells. |
| Target | FGF21 |
| Purity | Greater than 90% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Protein Sequence | MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPALPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS |
Storage & Handling
−| Storage | The lyophilized FGF-21 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted FGF-21 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing FGF-21 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Fibroblast growth factor 21, FGF-21
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Human FGF21 Protein, hFc Tag [orb1290908]
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 45.5 kDa after removal of the signal peptide. The apparent molecular mass of FGF21-hFc is approximately 40-55 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg Recombinant Human FGF21 Protein, N-Fc [orb2963293]
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>90% as determined by SDS-PAGE.
47.40 kDa
1 mg, 50 μg, 100 μgRecombinant Human FGF21 Protein, N-His-SUMO [orb2963518]
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>90% as determined by SDS-PAGE.
43.75 kDa
1 mg, 50 μg, 100 μgRecombinant Human FGF21 Protein, C-HSA-His [orb2963519]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
43.61 kDa
1 mg, 50 μg, 100 μg
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_061986.1 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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