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FGF-21 Recombinant Protein
Description
Research Area
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Activates ERK and FRS2alpha phosphorylation in bKlotho expressing cells. |
| Target | FGF21 |
| Tag | Fc Tag |
| Protein Sequence | Human FGF-21 (aa 1-209) is fused at the C-terminus to the Fc portion of human IgG1. |
| Purity | >95% (SDS-PAGE). Endotoxin level is less than 0.1EU/ μg purified protein (LAL test; Lonza). |
Storage & Handling
−| Storage | Working aliquots are stable for up to 3 months when stored at -20°C. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.2um-filtered solution in PBS. |
| Concentration | ~1mg/ml |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human FGF21 Protein, hFc Tag [orb1290908]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 45.5 kDa after removal of the signal peptide. The apparent molecular mass of FGF21-hFc is approximately 40-55 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μgRecombinant Human FGF21 Protein, N-Fc [orb2963293]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
47.40 kDa
1 mg, 50 μg, 100 μgRecombinant Human FGF21 Protein, C-HSA-His [orb2963519]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
43.61 kDa
1 mg, 50 μg, 100 μgRecombinant Human FGF21 Protein, N-His-SUMO [orb2963518]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
43.75 kDa
1 mg, 50 μg, 100 μg

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Protocol Information
FGF-21 Recombinant Protein (orb1230234)
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