Ferrocin B is an iron-containing cyclic decapeptide antibiotic isolated from the Pseudomonas fluorescens YK-310 strain. It exhibits strong antibacterial properties, primarily targeting Gram-negative bacteria, with a notable inhibitory effect against Pseudomonas aeruginosa. In murine infection models, the half-maximal effective dose (ED50) of Ferrocin B for P. aeruginosa is 0.593 mg/kg. Ferrocin B is applicable in anti-infective research studies.