FDW028

SKU: orb2567324

Description

FDW028 is a potent and selective inhibitor of fucosyltransferase 8 (FUT8). It exhibits anti-tumor activity by inducing B7-H3 degradation via defucosylation and chaperone-mediated autophagy pathways, making it a useful tool for studying metastatic colorectal cancer in both cellular and animal models.

Research Area

Cell Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 2768426-49-7
• MW: 388.47
• Purity: 99.55% (May vary between batches)
• Formula: C₂₂H₂₄N₆O
• SMILES: OC1=CC(=NC2=NC(=NN21)NCC3=CC=C(C=C3)CC)CNCC=4C=CC=CC4
• Target: Transferase,Autophagy,Akt,mTOR,B7
• Solubility: DMSO:80 mg/mL (205.94 mM)

Bioactivity

• Target IC50:
  SW480 cells:5.95 μM|HCT8 cells:23.78 μM
• In Vivo:
  METHODS: FDW028 (10, 20 mg/kg, intravenous injection, 4-6 weeks) was administered to athymic male SCID tumor xenograft mice to explore the effect of FDW028 on CRC growth. RESULTS:FDW028 exerted potent antitumor activity against CRC and significantly inhibited the growth of mouse tumors.
• In Vitro:
  METHODS: FDW028 (0.316-316μM, 72 hours) was used to treat SW480 and HCT-8 cells, and cell viability was detected with or without FUT8 knockdown. RESULTS:FDW028 exhibited potent antitumor capabilities in terms of cell proliferation, with half-maximal inhibitory concentration (IC50) values ​​of 5.95 µM and 23.78 µM in SW480 and HCT-8 cells, respectively. METHODS: SW480 and HCT-8 cells were treated with FDW028 (50 μM, 72 hours) for Transwell and wound healing assays. RESULTS:FDW028 significantly inhibited the migration of SW480 and HCT-8 cells.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Autophagy, FDW 028, FDW028, FUT8

Compound Identifiers

PubChem CID

53208107