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Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100 |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein of human Fascin |
| Target | FSCN1 |
| Molecular Weight | Calculated MW: 55 kDa; Observed MW: 55 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Immunocytochemistry analysis of Fascin (green) in 3T3 using Fascin antibody, and DAPI (blue).

Western blot analysis of Fascin in mouse skin, rat skin lysates using Fascin antibody.

Western blot analysis of Fascin in Hela, A549, HepG2, COS7 lysates using Fascin antibody.

Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Fascin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Protocol Information
Fascin Rabbit pAb (orb1565542)
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