FANCG Antibody

• Catalog Number: orb1537957
• Category: Antibodies
• Description: FANCG Antibody
• Target: FANCG
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Monkey
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence LEEFRTSLPKSCDL, from the C-Terminus of protein sequence according to NP_004620.1.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (3 µg/ml), Peptide-ELISA (1:32000), WB (0.3 - 1 µg/ml)
• Application notes: Further information: Immunohistochemistry: LS-B2997 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2997 was determined to be 3 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FANCG, DNA repair protein XRCC9, Fanconi anemia gr
• Note: For research use only

HEK293 overexpressing FANCG and probed with (mock transfection in first lane).