FANCE Antibody

• Catalog Number: orb1248909
• Category: Antibodies
• Description: FANCE Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Bovine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EHKSLESLADGGS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 70kDa band in Human Adipose and Uterus lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.7kDa according to NP_068741.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Placenta shows nuclear staining in some trophoblasts. Recommended concentration, 3-6ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FANCE
• UniProt ID: Q9HB96
• NCBI: NP_068741.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FANCE, Fanconi anemia, complementation group E, FA
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 70kDa band in Human Adipose and Uterus lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.7kDa according to NP_068741.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Placenta shows nuclear staining in some trophoblasts. Recommended concentration, 3-6ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248909 (3.8 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.