Factor XIII alpha antibody (MSVA-813R)

• Catalog Number: orb2276331
• Category: Antibodies
• Description: Factor XIII alpha antibody validated for immunohistochemistry on 76 different Normal Tissues
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant fragment of human Factor XIIIa protein (aa46-181) (exact sequence is proprietary)
• UniProt ID: P00488
• Tested applications: IHC
• Dilution range: 1:100-1:200
• Application notes: Positive Control: Colon: A fraction of monocytic cells in the mucosa should show a strong cytoplasmic factor XIIIa immunostaining. Negative Control: Colon: Factor XIIIa immunostaining should be absent in epithelial, endothelial and smooth muscle cells. Cellular Localization: Cytoplasmic and Secreted Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Clone Number: Biorbyt-813R
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Coagulation factor XIII A chain; Coagulation facto
• Note: For research use only

A fraction of monocytic cells in appendix mucosa shows a strong cytoplasmic factor XIIIa immunostaining.

Hodgkin‘s lymphoma containing a dense population of factor XIIIa positive cells.

Strong factor XIII immunostaining of sinus histiocytes in a lymph node.