F(ab')2 Rabbit IgG (H&L) Antibody Pre-Adsorbed

SKU: orb348321

Description

F(ab')2 Rabbit IgG (H&L) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
• Reactivity: Rabbit
• Application Notes:
  F(ab')2 Anti-Rabbit IgG Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Donkey
• Clonality: Polyclonal
• Isotype: IgG F(ab')2
• Immunogen: Rabbit IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rabbit IgG, and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat, and Sheep Serum Proteins.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Donkey F(ab')2 Anti-Rabbit IgG Pre-Adsorbed Antibody, Donkey Fab2 Anti-Rabbit IgG Antibody

ELISA results of purified Donkey F(ab')2 Anti-Rabbit IgG Antibody Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins tested against purified Rabbit IgG. Each well was coated in duplicate with 1.0 µg of Rabbit IgG (p/n orb2652747). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Goat anti-Donkey MX7 HRP conjugated, and TMB substrate (p/n orb348651).