F(ab')2 Human IgG (H&L) antibody

• Catalog Number: orb346777
• Category: Antibodies
• Description: F(ab')2 Human IgG (H&L) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG F(ab')2
• Immunogen: Human IgG whole molecule
• Concentration: 10.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: F(ab')2 Anti-Human IgG antibody is a F(ab’)2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit F(ab')2 anti-Human IgG Antibody, rabbit Fab
• Note: For research use only
• Application notes: F(ab')2 Anti-Human IgG antibody is suitable for ELISA, Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.