Fab Rabbit IgG (H&L) Antibody Biotin Conjugated

• Catalog Number: orb348492
• Category: Antibodies
• Description: Fab Rabbit IgG (H&L) antibody (Biotin)
• Clonality: Polyclonal
• Species/Host: Donkey
• Isotype: IgG Fab
• Conjugation: Biotin
• Reactivity: Rabbit
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
• Immunogen: Rabbit IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:75,000 - 1:150,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Fab Anti-Rabbit IgG Biotin Antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Donkey Fab Anti-Rabbit IgG Biotin Conjugated Antib
• Note: For research use only