EZH1 Antibody

SKU: orb420353

Description

Ezh1 antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 5.0 ug/ml, WB: 1.0 ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Affinity purified Anti-EZH1 antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~86 kDa in size corresponding to Ezh1 by western blotting in the appropriate cell lysate or extract. Testing using positive control NIH/3T3 nuclear extract p/n W10-001-A74.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Affinity purified Anti-EZH1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human EZH1 protein.
• Target: EZH1
• Purity: Anti-EZH1 is directed against human EZH1 at an internal position. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in mouse, bovine, and orangutan species based on 100% homology for the immunogen sequence.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.95 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-EZH1 antibody, KIAA0388, Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1, EZH-1, EZH 1

Western Blot of Rabbit anti-Ezh1 antibody. Marker: Opal Pre-stained ladder. Lane 1: NIH/3T3 WCL (p/n orb348714). Lane 2: NIH/3T3 nuclear extract (p/n orb348724). Load: 10 µg per lane. Primary antibody: Ezh1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:70000 for 60 min at RT. Blocking Buffer: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 86 kDa for Ezh1 in lane 2 NIH/3T3 nuclear extract (p/n orb348724).

Quick Database Links

Gene Symbol

EZH1

UniProt

Q92800

RefSeq (Protein):NP_001982.2