Extreme Thermolabile UNG (Uracil N-Glycosylase)

SKU: orb1821420

Description

Extreme Thermolabile UNG (UDG) is ideal for use in one-step RT-PCR systems to remove contaminations with uracil-containing DNA before onset of the reverse transcription. The enzyme is fully active at temperatures between 15 to 25°C and inactivated at 50°C. Extreme Thermolabile UNG is a recombinant uracil-DNA glycosylase from marine bacterium BMTU 3346. UNG catalyzes the hydrolysis of the N-glycosidic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. An amount of 1 unit UNG completely digests 104to 106copies of U-containing DNA fragments in 2 min at 50°C.

Key Properties

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Storage & Handling

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Storage	store at -20 °C. avoid freeze/thaw cycles
Form/Appearance	liquid
Concentration	1 unit/µl
Disclaimer	For research use only

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