Etomoxir sodium salt

SKU: orb1302778

Description

Etomoxir sodium salt is a potent and selective inhibitor of carnitine palmitoyltransferase 1A (CPT1A), effectively blocking fatty acid oxidation (FAO). This mechanism underlines its utility in metabolic research and investigations into its antitumor effects, both in vitro and in vivo, across various cancer models.

Research Area

Cell Biology

Key Properties

• CAS Number: 828934-41-4
• MW: 320.74
• Purity: 99.67%
• Formula: C₁₅H₁₈ClO₄.Na
• SMILES: [Na+].[O-]C(=O)[C@@]1(CCCCCCOc2ccc(Cl)cc2)CO1
• Target: Apoptosis
• Solubility: DMSO:3.21 mg/mL (10.01 mM);H2O:5 mg/mL (15.59 mM);Saline:3.5 mg/mL (10.91 mM)

Bioactivity

• Target IC50:
  Hepatocyte cells (rat):10 µM (fatty acid oxidation)|faTT cellsy acid oxidation in Hepatocyte cells (human):0.1 µM|KB cells:2.76 µM|faTT cellsy acid oxidation in Hepatocyte cells (guinea pig):1 µM
• In Vivo:
  METHODS: To test the antitumor activity in vivo, Etomoxir sodium salt (40 mg/kg) was intraperitoneally injected into BALB/c nude mice carrying human bladder cancer tumor T24 every two days for twenty days. RESULTS: Etomoxir significantly inhibited tumor growth. METHODS: To test the activity against multiple sclerosis, Etomoxir sodium salt (15 mg/kg) was intraperitoneally injected into a C57BL/6J mouse model of EAE induction once a week or every two days for two weeks. RESULTS: Etomoxir-treated mice showed reduced disease severity as well as reduced inflammation and demyelination. Disruption of fatty acid metabolism promotes downregulation of CNS inflammation and this metabolic pathway is a potential therapeutic target for multiple sclerosis.
• In Vitro:
  METHODS: BCa cell lines UM-UC-3 and T24 were treated with Etomoxir sodium salt (20-200 μM) for 24-72 h. Cell viability was examined using MTT. RESULTS: Etomoxir inhibited the viability of UM-UC-3 and T24 cells in a dose-dependent manner. METHODS: Human breast cancer cells MCF-7 and T47D were treated with Etomoxir sodium salt (0.1-50 μM) for 24 h. Cellular FAO activity was measured by 3H palmitic acid. RESULTS: Low micromolar concentrations of Etomoxir were sufficient to achieve maximal inhibition of FAO in MCF-7 and T47D cells.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CPT 1, CPT-1a, (R)-Etomoxir sodium salt, Apoptosis, carnitine, FAO, fatty, Etomoxir sodium, Etomoxir sodium salt, palmitoyltransferase, palmitate, Na, β-oxidation, oxidation

Compound Identifiers

PubChem CID

57345784