Estradiol

SKU: orb1309940

Description

Estradiol (E2) is a key steroidal estrogen vital for female reproductive health. It enhances IL-6 expression via estrogen receptor beta (ERβ). This product is used in research on endocrinology, immunology, and inflammation, applicable in both in vitro cell studies and in vivo model systems.

Research Area

Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 50-28-2
• MW: 272.38
• Purity: 98.25% (May vary between batches)
• Formula: C₁₈H₂₄O₂
• SMILES: C[C@@]12[C@]([C@]3([C@@](C=4C(CC3)=CC(O)=CC4)(CC1)[H])[H])(CC[C@@H]2O)[H]
• Target: Estrogen Receptor/ERR,Estrogen/progestogen Receptor,Endogenous Metabolite
• Solubility: Ethanol:20.4 mg/mL (74.9 mM);DMSO:250 mg/mL (917.84 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2.73 mg/mL (10.02 mM)

Bioactivity

• Target IC50:
  ERα:22-28 nM|ERα (mouse):22-28 nM|ERα (human):22-28 nM
• In Vivo:
  METHODS: To assay in vivo activity, Estradiol (0.2 mg/kg) and progesterone (5-20 mg/kg) were administered intraperitoneally as a single injection to ovariectomized C57BL/6 female mice. RESULTS: Mice receiving Estradiol alone or Estradiol plus 10-20 mg/kg progesterone showed significantly enhanced memory for new objects. METHODS: To assay in vivo activity, Estradiol (0.2 mg/kg) was administered by gavage to ovariectomized C57BL/6 female mice once daily for two weeks. RESULTS: Long-term activation of ERβ reduced hot flash-like symptoms and enhanced spatial and object recognition memory in ovariectomized mice.
• In Vitro:
  METHODS: Human lens epithelial cells HLEC were treated with Estradiol (0.001-10 μM) for 24 h. Cell viability was measured using the MTT. RESULTS: The number of viable cells was significantly reduced in the presence of 10 μM Estradiol. METHODS: Human breast cancer cells MB231 were treated with Estradiol (100 nM) for 24 h. Cell motility was detected using scratch/wound healing assay and transwell assay. RESULTS: Estradiol treatment reduced the migration and invasion of MB231 cells.
• Cell Research:
  Estradiol is dissolved in 0.01% ethanol. Briefly, B9 cells (5×103/well of a 96-well plate) are cultured with a series of dilutions of the supernatants in a final volume of 100 mL of RPMI 1640, supplemented with 5×10-5mol/Lof 2-mercaptoethanol, 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin in flat-bottom microtiter plates. After 42 h, 0.5 μCi of [3H]thymidine is added. 6 h later, the cells are harvested and the radioactivity incorporated is determined. IL-6 is quantitated from a standard curve set up with known amounts of recombinant human or mouse IL-6. The anti-IL-6 monoclonal antibody completely inhibited the ability of the B9 cells to proliferate in response to recombinant mouse IL-6. In addition, we determined that B9 cells do not proliferate in response to IL-I or TNF, and that antibodies to these two cytokines did not affect the cell proliferation in response to IL-6. Further, it is established that neither Estradiol (17β-estradiol), nor the 0.01% ethanol used as vehicle in these experiments, has any effect on the bioassay.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

beta-Estradiol, b-Estradiol, E2, E-2, E 2, 17beta-Estradiol, 17b-Estradiol, 17beta-Oestradiol, 17b-Oestradiol, 17β-Oestradiol, 17β-Estradiol, ERR, ER, Estradiol, Endogenous Metabolite, EndogenousMetabolite, Estrogen Receptor/ERR, EstrogenReceptor, Estrogen Receptor, Estrogen, human endometrial stem cells, Inhibitor, inhibit, neural differentiation, progestogenReceptor, progestogen Receptor, steroid sex hormone, uterine fibroids, β-Estradiol

Compound Identifiers

PubChem CID

450