erpA8 Antibody

SKU: orb344666

Description

Erpn/Ospe antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:5,000, WB: 1:1,000
• Reactivity: Bacteria
• Application Notes:
  Anti-ErpN/OspE antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at 17.1 kDa in size corresponding to ErpN/OspE by Western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi ErpN/OspE protein.
• Target: erpA8
• Purity: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. It is directed against, and shows specific reactivity for, Borrelia burgdorferi OspE protein. Reactivity with ErpN/OspE protein from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit anti-ErpN Antibody, rabbit anti-OspE Antibody, rabbit anti-ErpN/OspE Antibody, ErpA, erpA8 protein, outer surface protein E, Borrelia burgdorferi OspE, ospE/F-Related Protein N, BB_L39, cp32-8

Western blot showing detection of 0.1 µg recombinant proteins in Western blot. Lane 1: Molecular weight markers. Lane 2: MBP-ErpN/OspE fusion protein (arrow; 59.5 kDa expected MW). Lane 3: fusion protein (MBP-tagged) plus cleaved fusion proteins (without MBP). Lane 4: MBP alone. The lower bands are probably breakdown products. The upper bands in lane 3 are fusion protein (top band), or breakdown products of the fusion protein (bands in middle of blot). Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n orb348540, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n orb347654) secondary antibody was used at 1:40000 in orb348637 blocking buffer.

Quick Database Links

Gene Symbol

erpA8

UniProt

H7C7N5

RefSeq (Protein):WP_010883865.1