Ergosta-4,6,8(14),22-tetraen-3-one

SKU: orb1684622

Description

Ergosta-4,6,8(14),22-tetraen-3-one

Research Area

Cell Biology, Protein Biochemistry

Key Properties

• CAS Number: 19254-69-4
• MW: 392.62
• Formula: C₂₈H₄₀O
• SMILES: C[C@@]12C(=C3[C@@]([C@]4(C)C(C=C3)=CC(=O)CC4)(CC1)[H])CC[C@@]2([C@@H](/C=C/[C@@H](C(C)C)C)C)[H]
• Target: Others
• Solubility: DMSO:16 mg/mL (40.75 mM)

Bioactivity

• In Vivo:
  Ergosta-4,6,8(14),22-tetraen-3-one (10, 20 mg/kg, gavage, for 8 weeks) exhibited certain preventive effects on aristolochic acid I (AAI)-induced early kidney injury in rats. This compound significantly reduced serum creatinine, blood urea nitrogen, urinary protein, and urinary NAG levels, while also alleviating pathological damage in renal tissues .
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• In Vitro:
  Ergosta-4,6,8(14),22-tetraen-3-one (0, 5, 10, 15, and 20 μg/ml, for 6, 12, and 24 hours) exhibited significant cytotoxicity against HepG2 cells, with IC50 values decreasing as treatment duration and concentration increased, reaching 15.6, 11.8, and 10.0 μg/ml, respectively . Treatment with Ergosta-4,6,8(14),22-tetraen-3-one (0, 5, 10, and 20 μg/ml, for 12 hours) induced cell cycle arrest at the G2/M phase in HepG2 cells, with the proportion of G2/M phase cells peaking at 12 hours . Treatment with Ergosta-4,6,8(14),22-tetraen-3-one (0, 5, 10, and 20 μg/ml, for 12 hours) significantly increased apoptosis in HepG2 cells, with the proportions of early and late apoptotic cells rising in correlation with the concentration of Ergosta-4,6,8(14),22-tetraen-3-one . Treatment with Ergosta-4,6,8(14),22-tetraen-3-one (0, 5, 10, and 20 μg/ml, for 12 hours) led to the activation of caspase-3, -8, and -9, increased cleavage of PARP, upregulation of Bax protein expression, downregulation of Bcl-2 protein expression, and an elevated Bax/Bcl-2 ratio, indicating that ergone induces apoptosis in HepG2 cells through both intrinsic and extrinsic apoptotic pathways .
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

p53

Compound Identifiers

PubChem CID

6441416