Epirubicin HCl

SKU: orb1310913

Description

Epirubicin hydrochloride is an anthracycline derivative that acts as a topoisomerase II and Foxp3 inhibitor. It is widely used in cancer research for its antitumor activity, demonstrating efficacy in both in vitro cytotoxicity assays and in vivo tumor model studies.

Research Area

Cell Biology, Infectious Disease & Virology, Molecular Biology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 56390-09-1
• MW: 579.99
• Purity: >98%
• Formula: C₂₇H₂₉NO₁₁ · HCl
• SMILES: Cl.COc1cccc2C(=O)c3c(O)c4C[C@](O)(C[C@H](O[C@H]5C[C@H](N)[C@@H](O)[C@H](C)O5)c4c(O)c3C(=O)c12)C(=O)CO
• Target: Topoisomerase
• Solubility: Soluble in DMSO (up to 50 mg/ml), or in Water (up to 5 mg/ml).

Bioactivity

• Target IC50:
  Topoisomerase II:12 μM|synthesis and function of DNA:62.7 μM (in rat glioblastoma cell lines)|Hepatoma G2 cells proliferation:1.6 μg/mL (24h)
• In Vivo:
  METHODS: To test the antitumor activity in vivo, Epirubicin hydrochloride (7 mg/kg) was administered intravenously three times at four-day intervals to BALB/c nu/nu nude mice bearing MDA-MB-231 xenografts. RESULTS: Epirubicin reduced tumor growth by half and resulted in a more than 10% weight loss. the IR value of Epirubicin at 7 mg/kg was 53.3%.
• In Vitro:
  METHODS: Neuroglioma cells U-87 and neuronal primary cultures were treated with Epirubicin hydrochloride (0.5-100 µM) for 48 h. Cell viability was measured by MTT assay. RESULTS: Epirubicin significantly reduced the viability of rat U-87 cells in a concentration-dependent manner, with an IC50 of 6.3 µM. In contrast to U-87 glioma cells, rat normal neuronal cells were resistant to Epirubicin. METHODS: Human breast cancer cells MDA-MB-231 and SK-BR-3 were treated with Epirubicin hydrochloride (2-3 µM) for 48 h, and apoptosis was detected by Flow cytometry. RESULTS: Epirubicin significantly increased sub-G cells in G2/M block.
• Cell Research:
  Hep G2 cells (500 cells/well, monolayer) are plated in a 96-well plate. The next day the cells are treated with Epirubicin in the medium. At the end of the incubation periods, 15% volume of MTT dye solution is added. After 1 hr of incubation at 37℃, an equal volume of solubilization/stop solution (dimethylsul-foxide) is added to each well for an additional 1 hr incubation. The absorbance of the reaction solution at 570 nm is recorded.(Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cell, derivative, DNA/RNA Synthesis, DNA Synthesis, DNASynthesis, Apoptosis, 4'-Epidoxorubicin, 4'-epidoxorubicin HCl, 4'-Epidoxorubicin Hydrochloride, 4'-Epidoxorubicin hydrochloride, Epirubicin, Epirubicin HCl, Epirubicin hydrochloride, Epirubicin Hydrochloride, Foxp3, L-arabino, Inhibitor, inhibit, Pharmorubicin, Topo, Topoisomerase, RNA Synthesis, RNASynthesis

Compound Identifiers

PubChem CID

65348