EPCAM Antibody

SKU: orb1939252

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:8000, IHC-P-Leica - 1:1000
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: B685EV47X3X3
• Immunogen: This EPCAM antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 59-86 amino acids from human EPCAM. Antigen Region: 59-86 aa.
• Target: EPCAM
• Molecular Weight: 34932 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1

All lanes: Anti-EPCAM Antibody (N-term) at 1:8000 dilution. Lane 1: HT-29 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 39 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human appendix tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Quick Database Links

Gene Symbol

EPCAM

UniProt

P16422

RefSeq (Protein):NP_002345.2