Endomorphin 2

SKU: orb1299968

Description

Endomorphin-2 (EM-2) is an endogenous mu-opioid receptor (MOR) agonist that presynaptically and postsynaptically inhibits spinal motoneuron activity, as evidenced by reduced sEPSC frequency and amplitude. This peptide is used in neuroscience research to study opioidergic modulation of motor circuits in both in vitro spinal cord preparations and in vivo pain and motor control models.

Research Area

Metabolism Research, Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 141801-26-5
• MW: 571.67
• Purity: 99.64% (May vary between batches)
• Formula: C₃₂H₃₇N₅O₅
• SMILES: C(N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CC2=CC=CC=C2)C(N)=O)=O)(=O)[C@H]3N(C([C@H](CC4=CC=C(O)C=C4)N)=O)CCC3
• Target: Opioid Receptor
• Solubility: DMSO:10 mM

Bioactivity

• Target IC50:
  μ opioid receptor:20-30 nM(Ki)
• In Vivo:
  After surviving for 3 days, all the rats retrograde labeled by WGA-HRP were deeply anesthetized by injection of an overdose of sodium pentobarbital (100 mg/kg), and then perfused with 100 ml of 0.01 M PBS (pH 7.4), followed by 500 ml of a fixative consisting of 4% paraformaldehyde and 0.01% glutaraldehyde in 0.1 M PB (pH 7.4). The spinal cord were removed and stored in 0.1 M PB (pH 7.4) containing of 4% paraformaldehyde at 4°C for 2–4 h. The intact sections were chose for TMR and Endomorphin-2(EM-2) or MOR double staining. The sections were blocked with 10% normal goat serum in 0.01 M PBS (pH 7.4) and then incubated for 24 h with guinea pig anti-TMR (1:500) and rabbit anti-EM-2 (1:200) or rabbit anti-MOR (1:500) antibodies at room temperature (RT). After washing in 0.01 M PBS (pH 7.4), sections were incubated for 8 h at RT with: (1) biotinylated donkey anti-rabbit IgG (1:500) and Alexa594-conjugated donkey anti-guinea pig IgG (1:500) diluted with PBS-NDS (for TMR and EM-2 staining); (2) biotinylated donkey anti-rabbit IgG (1:500) and Alexa488-conjugated donkey anti-guinea pig IgG (1:500) diluted with PBS-NDS (for TMR and MOR staining). Then, after washing in 0.01 M PBS (pH 7.4), sections were incubated with FITC-Avidin (1:1,000) (for EM-2 staining) or Alexa594-Avidin (1:1,000) (for MOR staining) in 0.01 M PBS containing 0.5% Triton X-100 (PBS-X) for 3 h at RT. Finally, the sections were rinsed with 0.01 M PBS, mounted onto clean glass slides, air-dried and cover slipped with a mixture of 0.05 M PBS containing 50% (v/v) glycerin and 2.5% (w/v) triethylenediamine. Images were taken using a laser-scanning confocal microscope.

Storage & Handling

• Storage: keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

μ-opioid receptor, Inhibitor, inhibit, Opioid Receptor, OpioidReceptor, Endomorphin 2, Endomorphin2, Endomorphin-2

Compound Identifiers

PubChem CID

5311081