EN4

SKU: orb1298401

Description

EN4

Research Area

Cell Biology

Key Properties

• CAS Number: 1197824-15-9
• MW: 416.47
• Purity: 98.51% (May vary between batches)
• Formula: C₂₅H₂₄N₂O₄
• SMILES: CCOc1ccc(Oc2ccccc2NC(=O)c2ccc(CNC(=O)C=C)cc2)cc1
• Target: c-Myc
• Solubility: 10% DMSO+90% Corn Oil:3.3 mg/mL (7.92 mM);DMSO:245 mg/mL (588.28 mM)

Bioactivity

• In Vitro:
  EN4 (50 μM; for 60 hours) treatment significantly decreases the protein levels of representative MYC-regulated target genes, including CDK2 and CDC25A. EN4 treatment also substantially reduces MYC levels.EN4 shows the strongest inhibition of both MYC/MAX binding to its DNA consensus sequence in vitro as well as MYC transcriptional activity in cells. EN4 inhibited MYC/MAX binding to the E-box response element DNA consensus sequence in a dose-responsive manner with an IC50 value of 6.7 μM. EN4 also inhibits MYC luciferase reporter activity in a dose-responsive manner with an IC50 value of 2.8 μM.EN4 (1-50 μM; for 72 hours) treatment significantly impaires 231MFP breast cancer cell proliferation in a dosedependent manner, with >90% inhibition of proliferation at 50 μM.EN4 (50 μM; for 2 hours) treatment significantly reduced MYC thermal stability in 231MFP breast cancer cells.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

tumorigenesis, transcriptional, EN4, EN-4, EN 4, MYC, ligand, C171, c-Myc, cMyc, covalent

Compound Identifiers

PubChem CID

45917225