ELK1 Antibody

SKU: orb1273528

Description

ELK1 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Immunolabeling of the Elk-1 protein band in a Western blot of recombinant Elk-1 is blocked by the phosphopeptide used as antigen but not by the corresponding non-phospho-Elk-1. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Elk-1 (Ser383) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Ser383 of human Elk-1.
• Target: ELK1
• Molecular Weight: 46
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot of non-phospho- and phospho- recombinant Elk-1 showing specific immunolabeling of the ~46 Elk-1 protein phosphorylated at Ser383.

Quick Database Links

Gene Symbol

ELK1

UniProt

P19419

RefSeq:P19419