Human TIMP-1 ELISA Kit

SKU: orb3145546

Description

Human TIMP-1 ELISA Kit employs a two-site sandwich ELISA to quantitate Human TIMP-1.

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  Human TIMP-1 ELISA Kit employs a two-site sandwich ELISA to quantitate TIMP-1 in samples. An antibody specific for TIMP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP-1 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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Key Properties

• Reactivity: Human
• Sample Types: Cell culture supernatants#Serum#Plasma#Other biological fluids
• Range: 9.4 pg/ml-600 pg/ml
• Sensitivity: 9.4 pg/mL

Storage & Handling

• Storage: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
• Expiration Date: 6 months from date of receipt.
• Hazard Information: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
• Disclaimer: For research use only

Alternative Names

TIMP1; RP1-230G1.3; CLGI; EPA; EPO; FLJ90373; HCI; TIMP; erythroid potentiating activity;fibroblast collagenase inhibitor;Metalloproteinase inhibitor 1