EIF4E Antibody

SKU: orb1253729

Description

EIF4E Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human
• Application Notes:
  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: eIF4E (Phospho-Ser209) antibody was raised against a peptide sequence around phosphorylation site of serine 209 (S-G-S (p) -T-T) derived from Human eIF4E.
• Target: EIF4E
• Molecular Weight: 25 kDa
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CBP, EIF4F, AUTS19, EIF4E1, EIF4EL1, BCL2L3, mRNA cap-binding protein, eIF-4F 25 kDa subunit

Western blot analysis of lysed extracts from HT29 cells untreated or treated with Anisomycin using eIF4E (Phospho-Ser209).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209).

Quick Database Links

Gene Symbol

EIF4E

UniProt

P06730

RefSeq (Protein):NP_001124150.1