EIF3G Antibody

• Catalog Number: orb1257735
• Category: Antibodies
• Description: EIF3G Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human EIF3G (NP_003746.2).
• Concentration: batch dependent
• Dilution range: WB: 1:200 - 1:2000IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 42kDa
• Target: EIF3G
• UniProt ID: O75821
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: EIF3G, EIF3-P42, EIF3S4, eIF3-delta, eIF3-p44
• Note: For research use only
• Application notes: WB: 1:200 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EIF3G antibody (orb1257735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of U-2 OS cells using EIF3G Polyclonal Antibody (orb1257735) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.