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EIF2AK1 Rabbit Polyclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human EIF2AK1 |
| Target | EIF2AK1 |
| Protein Sequence | Synthetic peptide located within the following region: TCSDEFSSLRLHHNRAITHLMRSAKERVRQDPCEDISRIQKIRSREVALE |
| Molecular Weight | 71 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. Two isoforms at 71 kDa contain the peptide sequence and the protein is phosphorylated.

Sample Type: Human 293T, Antibody Dilution: 1.0 ug/ml. EIF2AK1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.

Sample Type: Human Hela, Antibody Dilution: 1.0 ug/ml. EIF2AK1 is strongly supported by BioGPS gene expression data to be expressed in HeLa.

Positive control (+): Hela (HL), Negative control (-): Human brain (BR), Antibody concentration: 1 ug/ml.

WB Suggested Anti-EIF2AK1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:62500, Positive Control: Human Lung.
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Protocol Information
EIF2AK1 Rabbit Polyclonal Antibody (orb578459)
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