Phospho-PKR (Thr451) Rabbit Polyclonal Antibody

SKU: orb6785

Description

Phospho-PKR (Thr451) Rabbit Polyclonal Antibody

Research Area

Kinases

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PKR around the phosphorylation site of Thr451 KG(p-T)LR
• Target: EIF2AK2
• Molecular Weight: 62 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

PKR(phospho T451); p-PKR(phospho T451); PKR(phospho-Thr451); double-stranded RNA-dependent Protein Kinase; interferon-induced, double-stranded RNA-activated protein kinase isoform a; protein kinase, interferon-inducible double stranded RNA dependent; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; p68 kinase; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; protein kinase RNA-activated; interferon-inducible RNA-dependent protein kinase; EIF2AK2; EIF2AK1; MGC126524; PKR; PRKRv.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-PKR (Thr451)) polyclonal Antibody, Unconjugated (orb6785) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Phospho-PKR (Thr451)) Polyclonal /Monoclonal Antibody, Unconjugated (orb6785) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-p-PKR (Thr451) (orb6785) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-p-PKR (Thr451) (orb6785) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD.

Quick Database Links

Gene Symbol

EIF2AK2

UniProt

P19525