E3330

SKU: orb1300433

Description

E3330 is a potent and selective small molecule inhibitor of the APE1/Ref-1 protein. By targeting APE1's redox function, it effectively suppresses NF-κB DNA-binding activity. This inhibitor is a valuable research tool for studying oxidative stress signaling, inflammation, and cancer biology in both cellular and animal models.

Research Area

Cell Biology

Key Properties

• CAS Number: 136164-66-4
• MW: 378.5
• Purity: >98%
• Formula: C₂₁H₃₀O₆
• SMILES: CCCCCCCCC\C(=C/C1=C(C)C(=O)C(OC)=C(OC)C1=O)C(O)=O
• Target: ROS,VEGFR,NF-κB,DNA/RNA Synthesis,HIF/HIF Prolyl-Hydroxylase,Reactive Oxygen Species
• Solubility: Soluble in DMSO (>50 mg/mL)

Bioactivity

• In Vivo:
  In mice with endotoxin-mediated hepatitis, E3330 (300 mg/kg, p.o.) attenuates the elevation of plasma tumor necrosis factor activity and protectes mice from liver injury. In Rat model, E3330 (100 mg/kg, p.o.) also protectes rats from severe liver injury induced with endotoxin plus galactosamine.
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• In Vitro:
  E3330 affects hemangioblast development in vitro via inhibition of Ape1 redox activity. E3330 inhibits the growth of human pancreatic cancer cell line PANC1, XPA1, MIAPACA, BxPC3, and PK9. E3330 also promotes exit of cell cycle in PANC1 cells, inhibits the DNA-Binding activity of HIF-1α and migration of pancreatic cancer cells. In JHH6 cells, E3330 prevents the functional activation of NF-κB via the alteration of APE1 subcellular trafficking and reduces IL-6 and IL-8 expression induced by TNF-α and FAs accumulation through blockage of the redox-mediated activation of NF-κB.
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• Cell Research:
  PANC1 cells are placed in one well of a 12-well plate and treated with 5 to 30 μM E3330. After a 24, 48, and 72 h of culture, the cells are washed with PBS and stained with trypan blue, and cell viability is examined by counting the live cell numbers.(Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

(2E)-3-[5-(2,3-Dimethoxy-6-methyl-1,4-benzoquinoyl)]-2-nonyl-2-propenoic acid

Compound Identifiers

PubChem CID

6439397