(E)-KPT330

SKU: orb1307341

Description

(E)-KPT330 is a selective CRM1 inhibitor that blocks nuclear protein export. This activity induces cell cycle arrest and apoptosis in mesothelioma cells in vitro and has shown antitumor efficacy in vivo xenograft models, supporting its investigation in cancer research.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 1421923-86-5
• MW: 443.31
• Purity: 99.49% (May vary between batches)
• Formula: C₁₇H₁₁F₆N₇O
• SMILES: FC(F)(F)c1cc(cc(c1)C(F)(F)F)-c1ncn(\C=C\C(=O)NNc2cnccn2)n1
• Target: CRM1
• Solubility: DMSO:4.44 mg/mL (10.02 mM)

Bioactivity

• In Vitro:
  As the Clinicalal candidate analog of KPT-185, KPT-330 exhibits similar effects on the viability of T-ALL cells and elicits rapid apoptotic response. KPT-330 also reduces cell growth in MOLT-4, Jurkat, HBP-ALL, KOPTK-1, SKW-3, and DND-41 cell lines, with IC50 values of 34-203 nM.
• Cell Research:
  Cell lines are cultured in RPMI 1640 medium, supplemented with 10% fetal bovine serum and penicillin/streptomycin. Cell Titer Glo assay is used to assess cell viability upon treatment with either dimethyl sulfoxide (DMSO) or KPT-330. Cells are plated at a density of 10 000 cells per well in a 96-well plate and incubated with DMSO or increasing concentrations of KPT-330. The cell viability is measured after 72 h exposure to KPT-330 and reported as a percentage of DMSO control cells. Jurkat cells that overexpress BCL2 are generated using MSCV-IRES-GFP retroviral expression system. Jurkat cells infected with BCL2 or control vector viruses are sorted by flow cytometry and the expression of BCL2 confirmed by Western blot analysis using BCL2 antibody.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CRM1, (E)-KPT 330, (E)-KPT330, (E)-KPT-330, (E)-RN, KPT330,, KPT330,(E) , KPT-330,(E)-, RN, XPO1

Compound Identifiers

PubChem CID

71493320