(E)-Ferulic acid

SKU: orb1301591

Description

(E)-Ferulic acid induces β-catenin phosphorylation and degradation, leading to upregulated Bax and downregulated survivin. This mechanism underlies its anti-proliferative and anti-migratory effects, as demonstrated in studies using the H1299 human lung cancer cell line for in vitro oncology research.

Research Area

Cell Biology, Metabolism Research, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 537-98-4
• MW: 194.18
• Purity: 99.63% (May vary between batches)
• Formula: C₁₀H₁₀O₄
• SMILES: C(=C/C(O)=O)\C1=CC(OC)=C(O)C=C1
• Target: Endogenous Metabolite,Wnt/beta-catenin,Ferroptosis,Bcl-2 Family
• Solubility: DMSO:65 mg/mL (334.74 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (10.3 mM)

Bioactivity

• In Vitro:
  trans-Ferulic acid exerted potent antioxidant effects. However, trans-Ferulic acid increased intracellular ROS levels, including hydrogen peroxide and superoxide anion, in H1299 cells. trans-Ferulic acid treatment inhibited cellular proliferation and induced moderate apoptotic cell death at the highest concentration used (0.6 mM). Furthermore, trans-Ferulic acid moderately inhibited the migration of H1299 cells at the concentrations of 0.3 and 0.6 mM and attenuated MMP-2 and MMP-9 activity. trans-Ferulic acid caused the phosphorylation of β-catenin, resulting in proteasomal degradation of β-catenin. Conversely, trans-Ferulic acid treatment increased the expression of pro-apoptotic factor Bax and decreased the expression of pro-survival factor survivin.
• Cell Research:
  The 2,2-diphenyl-1-picrylhydrazyl assay was used to determine free radical scavenging capability. Assessment of intracellular reactive oxygen species (ROS) was evaluated using oxidized 2',7'-dichlorofluorescin diacetate and dihydroethidium staining. Trypan blue exclusion, colony formation, and anchorage-independent growth assays were used to determine cellular proliferation. Annexin V staining assay was used to assess cellular apoptosis by flow cytometry. Wound healing and Boyden's well assays were used to detect the migration and invasion of cells. Gelatin zymography was used to detect matrix metalloproteinase (MMP-2 and MMP-9) activity. Western blotting was used to detect expression levels of various signaling pathway proteins.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Endogenous Metabolite, Coniferic acid, Beta catenin, betacatenin, beta-catenin, Bax, Bcl-2 Family, bcatenin, (E)Ferulic acid, (E)-Ferulic acid, (E)-Coniferic acid, (E) Ferulic acid, inhibit, Inhibitor, EndogenousMetabolite, Ferroptosis, Ferulic acid, Wnt/betacatenin, Wnt/b-catenin, Wnt, Wnt/β-catenin, β-catenin, βcatenin, trans-Ferulic acid

Compound Identifiers

PubChem CID

445858