E. coli Elongation Factor 4 Protein, His Tag (active enzyme)

SKU: orb3122855

Description

Elongation Factor 4 (EF4), also known as ​​LepA​​, is a highly conserved ​​GTPase translation factor​​ present across bacteria, mitochondria, and chloroplasts. It regulates protein synthesis by interacting with the ribosome during translation elongation. Unlike canonical elongation factors (e.g., EF-G) that promote forward translocation, EF4 catalyzes ​​back-translocation of tRNAs​​ on the ribosome, effectively acting as a molecular ’reverse gear‘ to reposition tRNAs and mRNA. This unique function allows EF4 to maintain translational fidelity under stress conditions (e.g., low pH or high ionic strength) by rescuing stalled ribosomes and correcting errors in protein synthesis.

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 68.5 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Key Properties

• Expression System: E. coli
• Target: Elongation Factor 4
• Reactivity: E. coli
• Tag: N-10×His
• Molecular Weight: 68.5 kDa
• Expression Region: Lys 2 - Lys 599
• Purity: 90%
• Conjugation: Unconjugated

Storage & Handling

• Storage: -70°C
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, 0.5 M NaCl, 1 M Arginine, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Elongation Factor 4

RefSeq:C4ZYJ2