DT3C Protein

SKU: orb1477852

Description

This DT3C Protein spans the amino acid sequence from region 33-417aa(P00588)+linker(GGGGSGGGGSGGGGS)+291-497aa(P19909). Purity: Greater than 90% as determined by SDS-PAGE.

Research Area

Epigenetics, Non-Animal

Images & Validation

• Application Notes:
  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

• Source: E.coli
• Biological Origin: Corynephage beta
• Biological Activity: ①After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies, cytotoxicity experiments were conducted on CHOK1/Human CCR8 cells. The ED50 is 0.3708-0.5608 μg/ml. ②After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies, cytotoxicity experiments were conducted on CT26/Human CCR8 cells. The ED50 is 0.4661-0.8614 μg/ml.
• Tag: N-terminal 6xHis-tagged
• Molecular Weight: 69.4 kDa
• Expression Region: 33-417aa(P00588)+linker(GGGGSGGGGSGGGGS)+291-497aa(P19909)
• Protein Length: Heterodimer
• Protein Sequence: GADDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDDDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELGLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEINFETRGKRGQDAMYEYMAQACAGNRVRRSVGSSLSCINLDWDVIRDKTKTKIESLKEHGPIKNKMSESPNKTVSEEKAKQYLEEFHQTALEHPELSELKTVTGTNPVFAGANYAAWAVNVAQVIDSETADNLEKTTAALSILPGIGSVMGIADGAVHHNTEEIVAQSIALSSLMVAQAIPLVGELVDIGFAAYNFVESIINLFQVVHNSYNRPAYSPGHKGGGGSGGGGSGGGGSIDEILAALPKTDTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGVWTYDDATKTFTVTE
• Purity: Greater than 90% as determined by SDS-PAGE.
• Endotoxins: Less than 1.0 EU/μg as determined by LAL method.

Storage & Handling

• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
• Form/Appearance: Lyophilized powder
• Buffer/Preservatives: Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies, cytotoxicity experiments were conducted on CHOK1/Human CCR8 cells. The ED50 is 0.3708-0.5608 μg/ml.

After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies, cytotoxicity experiments were conducted on CT26/Human CCR8 cells. The ED50 is 0.4661-0.8614 μg/ml.

Quick Database Links

UniProt

P19909, P00588