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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Immobilized recombinant Human TNFSF10 at 5ug/mL (100uL/well) can bind Human DR5, Fc Tag with a linear range of 0.4-13 ng/mL. |
| Target | TNFRSF10B |
| Tag | C-Fc Tag |
| MW | 40.5 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Ile 56 - Glu 182 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 50 mM Tris, 100 mM NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 16.4 KDa. Observed: 20-40 KDa, reducing conditions
20 μg, 100 μgRecombinant Mouse TRAIL R2 Protein [orb2994514]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 40.9 KDa. Observed: 50-75 KDa, reducing conditions
10 μg, 500 μg, 1 mg, 50 μgRecombinant Human TRAIL R2 Protein [orb2995140]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 15.3 KDa. Observed: 16-22 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Cynomolgus TNFRSF10B Protein [orb2993089]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 18 KDa. Observed: 22-30 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human TRAIL R2 Protein (Biotin) [orb2993682]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 17.1 KDa. Observed: 18-25 KDa, reducing conditions
20 μg, 100 μg

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The purity of rh TRAILR2 Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
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Protocol Information
DR5 Recombinant Protein (orb1227847)
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