Doxycycline hydrochloride

SKU: orb1227053

Description

Doxycycline Hydrochloride is a synthetic tetracycline derivative with similar antimicrobial activity.Doxycycline is a tetracycline antibiotic which is commonly used to treat a variety of infections.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 10592-13-9
• MW: 480.9
• Purity: >98% (HPLC)
• Formula: C₂₂H₂₄N₂O₈·HCl
• SMILES: O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])[C@@H](O)[C@]3([H])[C@@H](C)C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4)N.[H]Cl
• Target: MMP
• Solubility: DMSO: 3 mg/mL (6.23 mM)

Bioactivity

• In Vivo:
  Doxycycline hydrochloride (oral gavage; 200 or 800 mg/kg; once daily; 3 months) reduces MMP-9 activity in untreated HT mice in a dose-dependent manner. Animal model: 6-month-old female Heterozygous Col3a1-deficient (HT) mice. Dosage: 200 or 800 mg/kg. Administration: Oral gavage; 200 or 800 mg/kg; once daily; 3 months. Result: Reduced MMP-9 activity in a dose-dependent manner.
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• In Vitro:
  Doxycycline hydrochloride (0.01-10 μg/mL, 4 d) affects growth of glioma cells only under high concentrations. Doxycycline hydrochloride (0.01-10 μg/mL, 24 h) decreases MT-CO1 protein content with concentrations of 1 μg/mL and higher in SVG cells. Doxycycline hydrochloride (100 ng/mL, 1 μg/mL; 24 h) reduces proliferation of human cell lines. Doxycycline hydrochloride (0-250 μM, 72 h) inhibits cell viability of breast cancer cells. Cell Viability Assay Cell line: LNT-229, G55, and U343 glioma cells. Concentration: 0.01, 0.1, 1 or 10 μg/mL Incubation time: 4 days. Result: Affected growth of glioma cells only under high concentrations (10 μg/mL). Cell Viability Assay Cell line: SVG cells. Concentration: 0.01, 0.1, 1 or 10 μg/mL Incubation time: 24 hours. Result: Decreaseed MT-CO1 protein content with concentrations of 1 μg/mL and higher. Cell Proliferation Assay Cell line: MCF 12A, 293T cells. Concentration: 100 ng/mL, 1 μg/mL. Incubation time: 96 hours. Result: Caused reduced proliferation of MCF 12A and 293T cells at 1 μg/mL. Cell Viability Assay Cell line: MCF-7, MDA-MB-468 cells. Concentration: 0-250 μM. Incubation time: 72 hours. Result: Inhibited breast cancer cells in a dose-dependent manner with IC50 values for MCF-7 and MDA-MB-468 of 11.39 μM and 7.13 μM respectively.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Doxycycline Hydrochloride

Quick Database Links

Gene Symbol

MMP