DOTA-​NHS-​ester

SKU: orb1706414

Description

DOTA-​NHS-​ester

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 170908-81-3
• MW: 501.488
• Purity: 99.88% (May vary between batches)
• Formula: C₂₀H₃₁N₅O₁₀
• SMILES: O=C(ON1C(CCC1=O)=O)CN2CCN(CC(O)=O)CCN(CC(O)=O)CCN(CC(O)=O)CC2
• Target: PROTAC Linker,ADC Linker
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.99 mM);DMSO:50 mg/mL (99.7 mM)

Bioactivity

• In Vitro:
  METHODS: The binding kinetics of hIMB1636 to Trop2 antigen were measured by biofilm interferometry (BLI). Western blotting was used to screen Trop2 expression in pancreatic cancer cell lines. Flow cytometry and cell immunofluorescence were used to evaluate the binding ability of hIMB1636 and Trop2 on the cell surface. hIMB1636 was conjugated with p-SCN-Bn-NOTA (NOTA) and DOTA-NHS-ester (DOTA) for 64Cu and 177Lu radiolabeling. RESULTS According to the BLI results, hIMB1636 had a strong binding affinity to Trop2. According to the results of Western blotting, flow cytometry, and cell immunofluorescence, the T3M-4 cell line showed the strongest expression of Trop2 and the specific binding ability of hIMB1636. The radiochemical purity of 64Cu-NOTA-hIMB1636 and 177Lu-DOTA-hIMB1636 exceeded 95%.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

tumor, SKOV3, SPECT, tomography, single-photon, positron, PET, radiometal, inhibit, HSA, Inhibitor, emission, Antibody-drug conjugates linkers, cancer, ADC Linkers, ADCLinker, ADC Linker, DOTA​NHS​ester, DOTA-NHS-ester, DOTA ​NHS ​ester

Compound Identifiers

PubChem CID

11488945