DOT1L/DOT1 Antibody

• Catalog Number: orb1536259
• Category: Antibodies
• Description: DOT1L/DOT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-500 of human DOT1L (NP_115871.1). DHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRESKSNAATPTKGPEGKVAGPADAPMDSGAEEEKAGAATVKKPSPSKARKKKLNKKGRKMAGRKRGRPKKMNTANPERKPKKNQTALDALHAQTVSQTAASSPQDAYRSPHSPFYQLPPSVQRHSPNPLLVAPTPPALQKLLESFKIQYLQFLA
• Concentration: 0.72 mg/ml
• Dilution range: IHC, IHC-P (1:100), WB (1:500 - 1:2000)
• Conjugation: Unconjugated
• Target: DOT1L / DOT1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol. PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
• Alternative names: DOT1L, DOT1, DOT1-like protein, KMT4, Lysine N-met
• Note: For research use only
• Application notes: Further information: The predicted MW is 164kDa/184kDa, while the observed MW by Western blot was 160kDa.
• Expiration Date: 12 months from date of receipt.

Western blot of extracts of HeLa cell line, using DOT1L antibody.