KMT4 Rabbit Polyclonal Antibody

SKU: orb184545

Description

KMT4 Rabbit Polyclonal Antibody

Research Area

Cell Biology, DNA and RNA, DNA and RNA Researchh, Epigenetics, Epigenetics and Nuclear Signaling, Homelogous Recombination, Nucleotide excision repair (NER)

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Monkey, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human KMT4 (51-150/1739aa)
• Target: DOT1L
• Molecular Weight: 185 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Disrupter of telomere silencing protein 1; DOT 1; DOT1; DOT1 like; DOT1 like histone H3 methyltransferase(S. cerevisiae); DOT1 like histone H3 methyltransferase; DOT1 like protein; DOT1, S. cerevisiae, homolog of; DOT1L; EC 2.1.1.43; H3 K79 HMTase; Histone H3 K79 methyltransferase; Histone H3 methyltransferase; Histone H3 methyltransferase DOT1-like; Histone lysine N methyltransferase H3 lysine 79 specific; Histone methyltransferase DOT1L; KIAA1814; DOT1L_HUMAN; KMT4; Lysine N methyltransferase 4.

Sample: Liver (Mouse) Lysate at 40 ug, Liver (Rat) Lysate at 40 ug, Primary: Anti-KMT4 (orb184545) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 185 kD, Observed band size: 170 kD.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-KMT4 Polyclonal Antibody, Unconjugated (orb184545) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-KMT4 Polyclonal Antibody, Unconjugated (orb184545) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

DOT1L

UniProt

Q8TEK3