Donepezil HCl

SKU: orb1222816

Description

Donepezil is a selective and effective AChE inhibitor for bAChE and hAChE (IC50: 8.12/11.6 nM).

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 120011-70-3
• MW: 416
• Purity: >98% (HPLC)
• Formula: C₂₄H₂₉NO₃·HCl
• SMILES: Cl.COc1c(OC)cc2C(=O)C(CC3CCN(Cc4ccccc4)CC3)Cc2c1
• Target: bAChE| hAChE
• Solubility: H2O : 33.33 mg/mL 80.13 mM; DMSO : 6.2 mg/mL 14.91 mM

Bioactivity

• In Vivo:
  Animal model: Male imprinting control region (ICR) mice (6 weeks old). Dosage: 3-10 mg/kg. Administration: Administered orally. Result: Pretreatment with 3–10 mg/kg ameliorated scopolamine-induced memory impairment. Animal model: Hairless rats with an average weight of 300 g. Dosage: 3 and 10 mg/kg (Pharmacokinetic Analysis). Administration: Administered orally; and blood (250 μL) was collected through the tail vein. Result: After oral treatment (3 and 10 mg/kg), a maximum concentration (Cmax) was reached after approximately 1.2 ± 0.4 h and 1.4 ± 0.5 h, respectively, and gradually decreased.
• In Vitro:
  Cell Viability Assay Cell line: Cortical neuronal cells. Concentration: 0.01, 0.1, 1, and 10 μM Incubation time: 24 hours. Result: Exhibited significantly increased cell viability (maximized 89.2±2.1% in MTT, 96.3±5.5% in TBS, and 95.1±3.2% in CCK-8). Western blot analysis. Cell line: Cortical neuronal cells. Concentration: 10 μM. Incubation time: 24 hours before 20 μM Aβ42 exposure for 6 hours. Result: Effects of Donepezil on Akt and the GSK-3 signaling pathway were statistically significant in the presence of Aβ42 toxicity.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E2020

Quick Database Links

Gene Symbol

bAChE| hAChE