Dog Symmetric dimethylarginine (SDMA) ELISA Kit

SKU: orb782628

Description

Dog Symmetric dimethylarginine (SDMA) ELISA Kit

Research Area

Musculoskeletal & Connective Tissue Research, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog SDMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog SDMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog SDMA in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Canine
• Sample Types: cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.051 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Symmetric dimethylarginine ELISA Kit

Standard curve for orb782628 ELISA kit.

Quick Database Links

Gene Symbol

SDMA