Dog Osteocalcin (OC) ELISA Kit

SKU: orb781470

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin(OC).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog OC in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Canine
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.252 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

BGLAP ELISA Kit, OT ELISA Kit, BGP ELISA Kit, Bone Gla Protein ELISA Kit, Bone Gamma-Carboxyglutamate Protein ELISA Kit, Gamma-carboxyglutamic acid-containing protein ELISA Kit

Quick Database Links

UniProt

P81455