Dog Matrix Metalloproteinase 3 (MMP3) ELISA Kit

• Catalog Number: orb779630
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 3(MMP3).
• Tested applications: ELISA
• Reactivity: Canine
• Range: 1.57-100 ng/mL
• Assay Time: 3.5h
• Concentration: 100 ng/mL
• Sensitivity: 0.6 ng/mL
• Alternative names: SL1 ELISA Kit, STMY ELISA Kit, STMY1 ELISA Kit, ST
• Note: For research use only
• Application notes: standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog MMP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog MMP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog MMP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog MMP3 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Expiration Date: Please enquire.