Dog Matrix Metalloproteinase 3 (MMP3) ELISA Kit

SKU: orb779630

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 3(MMP3).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog MMP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog MMP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog MMP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog MMP3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Canine
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.6 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

SL1 ELISA Kit, STMY ELISA Kit, STMY1 ELISA Kit, STR1 ELISA Kit, Progelatinase ELISA Kit, Stromelysin 1 ELISA Kit, Transin-1 ELISA Kit