Dog Immunoglobulin E (IgE) ELISA Kit

SKU: orb1173607

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin E(IgE). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin E(IgE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin E(IgE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin E(IgE) in the samples is then determined by comparing the OD of the samples to the standard curve.

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog IgE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog IgE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog IgE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog IgE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Canine
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ug/mL
• Sensitivity: 0.062 ug/mL
• Concentration: 10 µg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

IGHE, Immunoglobulin Heavy Constant Epsilon, Ig epsilon chain C region