Dog High Sensitivity Cardiac Troponin T (hs-cTnT) ELISA Kit

• Catalog Number: orb782525
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitivity Cardiac Troponin T(hs-cTnT).
• Tested applications: ELISA
• Reactivity: Canine
• Range: 15.63-1000 pg/mL
• Assay Time: 3.5h
• Concentration: 1000 pg/mL
• Sensitivity: 5.32 pg/mL
• Alternative names: High Sensitivity Cardiac Troponin T ELISA Kit
• Note: For research use only
• Application notes: standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog hs-cTnT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog hs-cTnT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog hs-cTnT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog hs-cTnT in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.