Dog Glycated Hemoglobin A1c (HbA1c) ELISA Kit

SKU: orb1291282

Description

Dog HbA1c (Glycated Hemoglobin A1c) ELISA Kit

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 µg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog HbA1c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Canine
• Sample Types: plasma and erythrocyte lysates
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 15.63-1000 µg/mL
• Sensitivity: 4.07 µg/mL
• Concentration: 1000 µg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc