Dog Fibrillin 1 (FBN1) ELISA Kit

SKU: orb1291279

Description

The Dog Fibrillin 1 (FBN1) ELISA Kit is designed to quantify Fibrillin 1 (FBN1) in canine serum, plasma, tissue homogenates and other biological fluids. The kit has a detection range of 0.32-20 ng/mL and a sensitivity of 0.115 ng/mL.

Research Area

Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog FBN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog FBN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog FBN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog FBN1 in the samples is then determined by comparing the OD of the samples to the standard curve

  Read more...

Key Properties

• Reactivity: Canine
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.115 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

FBN, MASS, MFS1, OCTD, SGS, Asprosin