Dog Brain Natriuretic Peptide (BNP) ELISA Kit

SKU: orb781093

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Brain Natriuretic Peptide(BNP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Brain Natriuretic Peptide(BNP).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog BNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog BNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog BNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog BNP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Canine
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 2.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 9.83 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GC-B ELISA Kit, B-Type Natriuretic Peptide ELISA Kit, Ventricular Natriuretic Peptide ELISA Kit, Gamma-brain natriuretic peptide ELISA Kit, Brain natriuretic peptide 32 ELISA Kit

Quick Database Links

UniProt

P16859