Docosahexaenoic Acid

SKU: orb1301846

Description

Docosahexaenoic Acid (DHA) is an essential omega-3 fatty acid highly concentrated in the brain and retina, crucial for neurological development. It is widely used in neuroscience and cell biology research to study neuroprotection, visual function, and cellular mechanisms in both in vitro and in vivo models.

Research Area

Metabolism Research

Key Properties

• CAS Number: 6217-54-5
• MW: 328.49
• Purity: 99.89% (May vary between batches)
• Formula: C₂₂H₃₂O₂
• SMILES: CCC=CCC=CCC=CCC=CCC=CCC=CCCC(O)=O
• Target: Endogenous Metabolite
• Solubility: H2O:5 mg/mL (15.22 mM);Ethanol:30 mg/mL (91.33 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (12.18 mM);DMSO:100 mg/mL (304.42 mM)

Bioactivity

• Target IC50:
  HL-60 cells:74 μM|Bradykinin (RCASM cells):0.23 ± 0.03 μM (EC50)|DHL-4 cells:5.2 µM
• In Vivo:
  An experimental model of PD was created by four intraperitoneal injections of MPTP (4 × 20 mg/kg, at 12h intervals). Docosahexaenoic acid was given daily by gavage for 4 weeks (36 mg/kg/day). The number of apoptotic dopaminergic cells significantly increased in MPTP-treated mice compared to controls. Although DHA significantly diminished the number of cell deaths in MPTP-treated mice, it did not improve the decreased motor activity observed in the experimental PD model. Docosahexaenoic acid significantly diminished the amount of cell death in the MPTP+DHA group as compared to the MPTP group .
• In Vitro:
  When monocytes were differentiated to dendritic cells (DCs) in the presence of docosahexaenoic acid (DHA), the expression of costimulatory and antigen presentation markers was altered in a concentration-dependent way, positively or negatively, depending on the markers tested and the maturation stage of the DCs. DHA-treated, mature DCs showed inhibition of IL-6 expression and IL-10 and IL-12 secretion, and their lymphoproliferative stimulation capacity was impaired .

Storage & Handling

• Storage: store at low temperature,keep away from moisture,store under nitrogen | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cervonic acid, DHA, Docosahexaenoic Acid, inhibit, Inhibitor, EndogenousMetabolite, Endogenous Metabolite

Compound Identifiers

PubChem CID

3144